European Federation of Biotechnology

Question: Let's now look at a different PCR concept. Have you heard of colony PCR? How is it different from regulat PCR and when would you use it? Answer: Colony PCR is a high-throughput protocol used to find whether insert DNA is present or absent in a Plasmid construct (De Maeseneire, 2012). Using this process positive clone can easily be recognized. Conventional PCR is done to amplify copies of one or several pieces of DNA (Stohwasser, 2012). Several thousand copies of DNA can be amplified using the process. Colony PCR on the other hand is done to recognize correct ligation as well as products of plasmids. Colony PCR is generally used to specifically determine the number of active DNA inserts in Escherichia coli colonies. This is a specific only on colony forming microorganisms. References De Maeseneire, S., Soetaert, W., De Mey, M. (2012). Comparison of colony PCR methods for yeast. In1st Symposium on Applied Synthetic Biology in Europe(pp. 62-62). European Federation of Biotechnology (EFB). Stohwasser, R., Giebel, L. B., Raab, K., Bautz, E. K. F. (2012). Polymerase Chain Reaction.Diagnosis of Human Viruses by Polymerase Chain Reaction Technology,1, 372.